How do Sliver nanoparticles work to kill bacteria and reduce odors

Silver nanoparticles work by targeting odour causing bacteria and eliminating 99.9999% of them in a safe, effective and long lasting way. They also help wound and irritations to heal faster.

Why should a shampoo pH be different for each gender?

Skin pH differs between sex (higher in males) and that’s why our products are gender specific. An intact skin plays a significant role in the pathogenesis of several diseases and alterations in cutaneous pH due to the frequent use of an inappropriate shampoo can have a negative effect in its integrity. Bathing with a non – irritating shampoo and lukewarm water is proven to improve skin and coat hygiene and care. Beside this, physical removal of surface allergens and microbes combined with the rehydration of the stratum corneum is beneficial for a direct soothing effect to the skin. Using a mild to the skin, pH – balanced shampoo, helps the owner to preserve pet’ s skin and coat healthy, when utilized either as part of a therapeutic plan or in daily care.
[1] Cobiella D, Archer L, Bohannon M, Santoro D.Pilot study using five methods to evaluate skin barrier function in healthy dogs and in dogs with atopic dermatitis.Vet Dermatol .2019. [Epub ahead of print].
[2] Matousek JL, Campbell KL.A comparative review of cutaneous pH.Vet Dermatol .2002;13: 293 – 300.
[3] Matousek JL, Campbell KL, Kakoma I, Solter PF, Schaeffer DJ.Evaluation of the effect of pH on in vitro growth of Malassezia pachydermatis.Can J Vet Res .2003;67: 56 – 59.
[4] Olivry T, DeBoer DJ, Favrot C, Jackson HA, Mueller RS, Nuttall T, Prélaud P.International Task Force on Canine Atopic Dermatitis.Treatment of canine atopic dermatitis: 2010 clinical practice guidelines from the International Task Force on Canine Atopic Dermatitis.Vet Dermatol .2010;21: 233 – 248.
[5] Ruedisueli FL, Eastwood NJ, Gunn NK.et al.The measurement of skin pH in normal dogs of different breeds.In: Advances in Veterinary Dermatology.Kwochka KW, Willemse T, Von Tscharner C(eds).Butterworth – Heineman Oxford, 1998, pp .521– 522.

How do NanoSanitas products contribute to the wound healing process?

Wound healing is a complex biological process that occurs as a mean of maintaining the body and undergoes various processes that include, among many, inflammation. The topical application of silver nanoparticles contributes to the treatment of inflammation due to their excellent antimicrobial properties and thus, accelerate the wound healing process. For thousands of years, silver has been used as a healing agent by civilizations throughout the world. Hippocrates, the father of modern medicine, used silver due to its beneficial therapeutic effect and antibacterial properties and this has been confirmed repeatedly through literature. Our proprietary NanoSilver technology has been tested preclinically by the group of Veterinary Assoc. Prof. N. Diakakis and of Prof. L. Papazoglou, for the treatment of inflammations providing excellent wound healing properties (furthered by NSRF grant no.T2EDK – 02826). Thus, our carefully engineered nanoparticles can work as part of a holistic approach to accelerate wound healing and tissue regeneration.

 [1] Konop M, Damps T, Misicka A, Rudnicka L.Certain Aspects of Silver and Silver Nanoparticles in Wound Care: A Minireview.Journal of Nanomaterials .2016.
[2] Nam G, Purushothaman B, Rangasamy S, Song JM.Investigating the versatility of multifunctional silver nanoparticles: preparation and inspection of their potential as wound treatment agents.Int Nano Lett .2016; 6: 51 – 63.
[3] Paladimi F, Pollini M.Antimicrobial silver nanoparticles for wound healing application: Progress and future trends.Materials .2019;12.

Why does Multi-purpose fur & skin care spray contain sunscreen?

Just like humans, our pet’s skin can get irritated or even burned from sun exposure, especially those with white or short fur and light skin.Sun – sensitive areas of the body, such as the nose, around the lips, tips of the ears and the belly, can be easily damaged by the sun(usually manifesting in discoloration or red – spots) and thus, they require protection. The Multi – purpose fur and skin care spray will, not only protect our pets from UV rays(broad – spectrum sunscreen), but also moisturize their fur and sooth their skin due to natural ingredients like coconut oil and aloe vera.

What does the claim “helps against ticks & fleas” mean?

Ticks serve as vectors of a wide range of infectious agents deleterious to humans and animals. Several studies have proved the effect of silver nanoparticles against ticks or major pathogens transmitted by the bites of ticks, such as bacteria, viruses or protozoa. The efficacy of our silver nanoparticles has been tested by the group of Veterinary Prof. H. Papadopoulos against well – known parasites, indicating their potential as active ingredients in this area(furthered by NSRF grant no.T2EDK – 02826). Therefore, our carefully engineered silver nanoparticles can work as part of a holistic approach in order to protect our pets against pathogens and parasites.
[1] Benellia G, Maggic F, Romanob D, Stefaninib D, Vaseeharane B, Kumarf S, Higuchig A, Alarfajh AA, Mehlhorni H, Canale A.Nanoparticles as effective acaricides against ticks – A review.Ticks and Tick – borne Diseases .2017.
[2] Durán N, Durán M, de Souza CE.Silver and Silver Chloride Nanoparticles and their Anti – Tick Activity: a Mini Review.J.Braz.Chem.Soc .2017;28(6): 927 – 932.

Are these products Eco friendly?

All our products contain carefully selected worry-free ingredients (see below), found in premium human cosmetics. Our manufacturing process is eco – friendly and in accordance with international standards (ISO 9001 & ISO 22716), with no hazardous waste material dispersed into the environment as a by – product of the Nanomaterials production.

What are the ingredients of each product?

Shampoo Female Advanced Fur Care: Aqua (Water), Sodium Laureth Sulfate, PEG-4 Rapeseedamide, Cocamidopropyl Betaine, Glycerin, Sodium Chloride, PEG – 12 Dimethicone, Glycol Distearate, Glycereth – 2 Cocoate, Lactic Acid, Polyquaternium – 10, Butylene Glycol, Calendula Officinalis Flower Extract, Tetrasodium EDTA, Citric Acid, Parfum(Fragrance), CI 15985, CI 17200, Silver Nanoparticles.

Shampoo Female Skin Care: Aqua(Water), Sodium Laureth Sulfate, PEG – 4 Rapeseedamide, Cocamidopropyl Betaine, Glycerin, PEG – 7 Glyceryl Cocoate, Sodium Chloride, Polyquaternium – 10, Glycol Distearate, Glycereth – 2 Cocoate, Lactic Acid, Butylene Glycol, Calendula Officinalis Flower Extract, Tetrasodium EDTA, Citric Acid, Parfum(Fragrance), CI 15985, CI 17200, Silver Nanoparticles.

Shampoo Male Advanced Fur Care: Aqua(Water), Sodium Laureth Sulfate, PEG – 4 Rapeseedamide, Cocamidopropyl Betaine, Glycerin, Sodium Chloride, PEG – 12 Dimethicone, Glycol Distearate, Glycereth – 2 Cocoate, Lactic Acid, Polyquaternium -10, Butylene Glycol, Calendula Officinalis Flower Extract, Tetrasodium EDTA, Citric Acid, Parfum(Fragrance), CI 42090, CI 60730, Silver Nanoparticles. Shampoo

Male Skin Care: Aqua(Water), Sodium Laureth Sulfate, PEG – 4 Rapeseedamide, Cocamidopropyl Betaine, Glycerin, PEG – 7 Glyceryl Cocoate, Sodium Chloride, Polyquaternium – 10, Glycol Distearate, Glycereth – 2 Cocoate, Lactic Acid, Butylene Glycol, Calendula Officinalis Flower Extract, Tetrasodium EDTA, Citric Acid, Parfum(Fragrance), CI 42090, CI 60730, Silver Nanoparticles.

Shampoo Puppies: Aqua(Water), Sodium Laureth Sulfate, PEG – 4 Rapeseedamide, Cocamidopropyl Betaine, Glycerin, PEG – 7 Glyceryl Cocoate, Sodium Chloride, Sodium Lauroamphoacetate, Glycol Distearate, Glycereth – 2 Cocoate, Lactic Acid, Butylene Glycol, Chamomilla Recutita(Matricaria) Flower Extract, Tetrasodium EDTA, Citric Acid, Parfum(Fragrance), Silver Nanoparticles.

Multi – purpose Fur & Skin Care: Aqua(Water), Octocrylene, C12 – 15 Alkyl Benzoate, Panthenol, Cocos Nucifera(Coconut) Oil, Glycerin, Butyl Methoxydibenzoylmethane, Polyglyceryl – 6 Stearate, Polyglyceryl – 6 Behenate, Triethanolamine,Parfum(Fragrance), Tocopheryl Acetate, Disodium EDTA, Acrylates / C10 – 30 Alkyl Acrylate Crosspolymer, Xanthan Gum, Aloe Barbadensis Leaf Juice Powder, Silver Nanoparticles.

Odor – Vanish: Aqua(Water), Silver Nanoparticles.

Liquid Gold Serum: Cyclopentasiloxane, Dimethiconol, C12-15 Alkyl Benzoate, Silica Dimethyl Silylate, Argan Oil, Ethyl Ester of Hydrolyzed Silk, Parfum(fragrance), CI 77891, CI 77491, Synthetic fluorphlogopite, Triethoxycaprylylsilane, Gold NanoparticlesCyclopentasiloxane, Dimethiconol, C12 – 15 Alkyl Benzoate, Silica Dimethyl Silylate, Argan Oil, Ethyl Ester of Hydrolyzed Silk, Parfum(fragrance), CI 77891, CI 77491, Synthetic fluorphlogopite, Triethoxycaprylylsilane, Gold Nanoparticles.

We understand Nano Sanitas products are Parabens free, what is used as preservative?

Our current products are preservative-free and thus paraben-free without of course risking the contamination and growth of harmful bacteria. Silver nanoparticles prevent the growth of pathogens, thus maintaining and preserving the product for a long period of time. All our products have already undergone rigorous testing, such as stability, compatibility and challenge tests to assure safety and the long-term preservation of our products

Does Nano Sanitas can kill only single bacteria or all types of bacteria?

The antimicrobial activity of Silver Nano Particles (AgNPs) has been tested against both, MDR (multidrug-resistant) and non-MDR strains of bacteria. AgNPs have displayed synergistic antimicrobial effect when used in combination with antibiotics against both gram (+) and gram (-) bacteria, including methicillin resistant S. aureus (MRSA), methicillin-resistant Staphylococcus epidermidis (MRSE). Besides potency against MDR and non-MDR bacterial strains, AgNPs also act as a potent, fast-acting anti-fungal and anti-viral agent against a wide range of fungal and viral genera. In accordance with literature, we have tested Nano Sanitas grooming products against well established skin pathogens of the dog and cat: Staphyloccoci, Escherichia coli, Pseudomonas aeruginosa. AgNPs have a very broad-spectrum action without causing any kind of microbial resistance. It becomes obvious that AgNPs can be used against a wide range both gram (+) and gram (-) bacteria, fungi and viruses but not against all types of pathogens. Such action could only be expected form harsh detergents intended for industrial use. After all, there is no antibiotic of disinfectant currently on the market that is intended for human or animal use that has a claim against all types of pathogens.

Does NanoSanitas shampoo cause eye irritation?

NanoSanitas grooming products are carefully designed to optimize skin protection and fur care. Our uniquely formulated pH balanced products are enriched with natural oils and extracts in order to minimize the risk of ocular irritation. However, the addition of surfactants might still cause some minimum degree conjunctival irritation. Unfortunately, not all dogs will fully cooperate with their owner to thoroughly rinse their eye with tap water, thus some foam may remain underneath the eyelid, causing them to rub their eyes further establishing a vicious circle.
Even though all our products are carefully designed to be skin, eye and mucous friendly, we recommend to avoid eye and mucous contact in order to further minimize any potential cases or irritation.

How does the Silver in the Odor Vanish Spray reduce or eliminate odors?

Silver nanoparticles (Ag NPs) have been incorporated into foot powders, sprays, soaps, socks, shoe insoles and a wide range of fabrics used for pajamas, briefs and undergarment sand pillows. In recent years a wide range of other applications in medicine, such as wound dressings have also been developed. Silver is a proven natural antibacterial agent, removing malodors in killing bacteria that cause them. [1] That said, Nano Silver does not eliminate odors of other origin, e.g. ammonia in pets’ urea and thus, may in some cases seem to work more effectively than others.
Our Anti-odor spray does not absorb or mask the odor, but eliminates malodors from almost all types of substrates, such as fabrics, leather and tiles, while disinfecting them at the same time. Concerning the proper use, we suggest shaking before use and spraying at a distance of 30-50cm on pet accessories. Since inhibition of the odor depends on the microbial load, the number of sprayings should be evaluated accordingly.
Please find below some relevant articles concerning the anti-odor effect of silver nanoparticles on various types of textiles (widely used in pet accessories) and the mode of action regarding their antimicrobial activity.

  • “Βacteria, viruses, fungi and other mono-cellular microorganisms has been effectively controlled, using Ag NPs. They inhibited the reproduction and growth of those bacteria and fungi responsible for the infection, bad odor, itchiness and sores. Ag NPs are found to be highly efficient, fast acting, deodorizing, nontoxic, non-stimulating, non-allergic, tolerance free, hydrophilic and thus very effective for bacterial resistance.” [2]
  • “Nanosilver applications have been implicated in paint, plastic, textile and medical industries. Nanosilver particles absorb sweat and in turn avoid pungent smell of feet. Mashat et al. determined the antibacterial activity of Ag- coated fabrics and compared their efficacy in reducing foot-borne resident bacteria. The results have shown that coating of fabrics with Ag NPs can have an anti-odor effect and can be used to combat foot-borne bacteria.” [3]
  • “Velmurugan et al. have evaluated the antibacterial properties of synthesized Ag NPs onto cotton fabrics and leather against odor- or skin infection-causing bacteria. Silver nanoparticles presented to be a novel material whose antibiotic properties can be integrated into cotton fabric and leather products to prevent skin infections or odor.” [1]
  • “The process of wound healing is often accompanied by bacterial infection or critical colonization, resulting in protracted inflammation, delayed reepithelization, and production of pungent odors. The malodor produced by these wounds may lower health-related quality of life and produce psychological discomfort and social isolation. Many scientists have succeeded to reduce bacterial activity within wound sites and malodor, using nano-sized silver particles in wound care dressings. [4-7]
[1] Velmurugan P, Lee SM, Cho M, Park JH, Seo SK, Myung H, Bang KS, Oh BT. Antibacterial activity of silver nanoparticle-coated fabric and leather against odor and skin infection causing bacteria. Appl Microbiol Biotechnol. 2014 Oct;98(19):8179-89.[2] Deshmukh SP, Patil SM, Mullani SB, Delekar SD. Silver nanoparticles as an effective disinfectant: A review. Mater Sci Eng C Mater Biol Appl. 2019;97:954-965.[3] Mashat B, Awad A. H. A. The Impact of Nanosilver Particles on Foot -Odor Producing Microbes. Curr World Environ 2014; 9 (3).[4] Cho KH, Park JE, Osaka T, Park SG. The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochim Acta. 2005;51(5):956–960.[5] Percival SL, Bowler PG, Russell D. Bacterial resistance to silver in wound care. J Hosp Infect. 2005;60(1):1–7.[6] Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E-coli as a model for Gram-negative bacteria. J Colloid Interface Sci. 2004;275(1):177–182.[7] Pankongadisak P, Ruktanonchai UR, Supaphol P, Suwantong O. Development of silver nanoparticles- loaded calcium alginate beads embedded in gelatin scaffolds for use as wound dressings. Polym Int. 2015;64(2):275–283).

Are the ingredients tested on animals at all? Do you have an animal testing policy?

Nanosanitas research team has developed all our products driven by our love and respect towards the animals and the environment we all live in. Our ultimate goal is to promote their welfare by healing and protection. To accomplish that, we are in line with the Regulation (EC) No 1223/2009 of the European Parliament and of the Council of 30 November 2009 on cosmetic products. The directive establishes prohibition to test finished cosmetic products and cosmetic ingredients on animals. The ingredients contained in our formulas are either CPNP (Cosmetic Products Notification Portal) approved raw materials, which means that they will cause no harm either to the pet, humans or to the environment or were complied with extensive toxicological, antimicrobial and other testing that have preceded launching and were all approved by a bioethics committee.

How often do you recommend as a brand to wash our dogs?

Unless there is a medical reason for therapeutic baths, most dogs should be bathed once or twice a month. Of course, bath frequency greatly varies based upon their breed, lifestyle, length of coat, and how much homework a pet owner is willing to do. However, Nanosanitas grooming products are specially designed to fit every need, so if your dog’s habits or other medical reasons call for frequent use, you should be confident that our products are suitable for frequent use.

I have seen some brands using silver to keep the coat white and nice. Does the Silver nanoparticles in the shampoo also work as whitening for dogs with white coat?

Various shampoos and other products promise to enhance the coat colour of our dog, leaving it sleek and shiny. But, why in fact does our pet’s coat change colour? Discolouration itself does not cause any harm. However, it can be a symptom of an underlying medical problem. Medical issues concerning the eyes, the oral cavity, and also allergies or other inflammatory skin conditions that cause itching can cause coat staining. In all instances, colour discoloration is not just a cosmetic problem.
Strong detergents acting against excessive amounts of grease and dirt in the coat is not an option, as they may exacerbate an already existing skin condition. The addition of colour enhancement chemicals to either stain or whiten the coat, will just sweep the problem under the carpet by concealing an underlying medical condition. A balanced diet and appropriate hydration of the skin and coat is the key to a healthy and shiny coat.
Nanosanitas product line, represents a holistic and completely safe, chemical-free approach for healthy skin and sleek coat. The product line is hypoallergenic, pet, human and eco- friendly and includes a shampoo and a spray for regular use on the pet’s fur. Thanks to their unique, silver nanoparticle enhanced composition, the two products are designed to act synergistically in multiple ways. The line’s special shampoo is pH-balanced, specifically adapted for the different needs of male and female dogs making it completely skin-friendly. Silver nanoparticles act in many ways: 1) prevention of tick and fleas, 2) promotes healing and acts against bacteria and yeasts,3) eliminates odors related to skin diseases, 4) provide UV protection and contain hair conditioner for easy detangling and healthier hair and skin. Moreover, the products’ formulation with plant extracts provide gentle moisturizing and effective cleansing. The line’s unique formulation help relieve symptoms associated with inflammation, pruritus, malodor and skin infection, leading to overall skin condition improvement and less commonly colour staining occurring conditions.
Colour enhancing products do not represent a proper management regarding colour staining problems. Veterinarians’ consultations along with a balanced diet and regular use of gentle, balanced products for regular grooming are the key to a healthy, award winning skin and coat.